human cervical cancer cell lines c33a hpv negative (ATCC)
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Human Cervical Cancer Cell Lines C33a Hpv Negative, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1128 article reviews
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1) Product Images from "Sine oculis homeobox homolog 1 promotes DNA replication and cell proliferation in cervical cancer."
Article Title: Sine oculis homeobox homolog 1 promotes DNA replication and cell proliferation in cervical cancer.
Journal: International journal of oncology
doi: 10.3892/ijo.2014.2510
Figure Legend Snippet: Figure 1. SIX1 expression is increased by HPV oncoprotein in cervical cancer. (A) Immunohistochemical analysis of SIX1 protein in tissue microarray of human specimens. Representative samples of SIX1 staining are shown at x200 magnification (left). Bar, 100 µm. The immunohistochemical scores (HSCORE) of SIX1 are also shown (right). (B) The expressions of SIX1 in three cervical cancer cell lines were detected by western blot analysis. (C) The expressions of HPV-E6, HPV-E7 and SIX1 in indicated C33a cells were detected by western blot analysis. (D) At 48 h after HPV-E7 siRNA transfection, the expressions of HPV-E7 and SIX1 in cells detected by western blot analysis. *P<0.05; **P<0.01; ***P<0.001.
Techniques Used: Expressing, Immunohistochemical staining, Microarray, Staining, Western Blot, Transfection
Figure Legend Snippet: Figure 3. SIX1 regulates the expression of genes responsible for DNA replication. (A and B) In C33a-3.1 and C33a-SIX1 cells, SIX1 expression was detected by (A) western blot analysis, and the expression levels of indicated genes were detected by (B) real-time RT-PCR. (C and D) Two independent shRNAs were used to knockdown SIX1 expression in Caski cells. SIX1 expression was detected by (C) western blot analysis and the expression levels of indicated genes were detected by (D) real-time RT-PCR. *P<0.05; **P<0.01; ***P<0.001.
Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Knockdown